multilocus variable-number tandem-repeat analysis for genotyping of shigella sonnei strains isolated from pediatric patients
نویسندگان
چکیده
aim: the aims of this study were to characterize iranian shigella sonnei strains isolated from pediatric cases and evaluate the utility of multilocus variable-number tandem-repeat (vntr) analysis (mlva) for genotyping of local s. sonnei strains. background: s. sonnei has become the dominant species in certain parts of iran. although pfge is still a gold standard for genotyping and source tracking of food-borne pathogens, it is laborious, expensive, time-consuming, and often difficult to interpret. however, mlva is a pcr-based method, which is rapid, relatively inexpensive and easy to perform. patients and methods: a total of 47 s. sonnei isolates were obtained from sporadic cases of pediatric shigellosis in tehran, iran, during the years 2002-2003 (n=10) and 2008-2010 (n=37). the patients suffered from acute diarrhea and had evidence of more than three episodes of watery, loose, or bloody stools per day. a mlva scheme based on 7 vntr loci was established to assess the diversity of 47 s. sonnei isolates. results: based on the results, it was clear that the s. sonnei isolates were heterogeneous. overall, 47? s. sonnei isolates were discriminated into 21 different genotypes. analysis of the mlva profiles using a minimum spanning tree (mst) algorithm showed the usefulness of the mlva assay in discriminating s. sonnei isolates collected over different time periods. however, no correlation was found between the mlva genotypes and age, gender or clinical symptoms of the patients. conclusion: it is assumed that our s. sonnei isolates are derived from a limited number of clones that undergo minor genetic changes in the course of time. the present study has provided some valuable insights into the genetic relatedness of s. sonnei in tehran, iran.
منابع مشابه
Multilocus variable-number tandem-repeat analysis for genotyping of Shigella sonnei strains isolated from pediatric patients
AIM The aims of this study were to characterize Iranian Shigella sonnei strains isolated from pediatric cases and evaluate the utility of multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) for genotyping of local S. sonnei strains. BACKGROUND S. sonnei has become the dominant species in certain parts of Iran. Although PFGE is still a gold standard for genotyping and source track...
متن کاملDiversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated From Pediatric Patients
Multilocus variable number tandem repeat (VNTR) analysis (MLVA) is a new typing method with several advantages compared to other methods. Dissemination of Shigella is highly significant in developing countries. Whilst Shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in Iran, little is known about the genetic diversity of the local strains. Therefore, th...
متن کاملMultilocus variable-number tandem-repeat analysis for molecular typing of Shigella sonnei.
A multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) method was developed and evaluated for the subtyping of Shigella sonnei isolates. A total of 26 VNTR loci were identified by exploring the repeat sequence loci in the genomic sequences of S. sonnei strains Ss046 and 53G and by testing 536 isolates that had previously been characterized by pulsed-field gel electrophoresis (PFGE). ...
متن کاملdiversity of variable number tandem repeat loci in shigella species isolated from pediatric patients
multilocus variable number tandem repeat (vntr) analysis (mlva) is a new typing method with several advantages compared to other methods. dissemination of shigella is highly significant in developing countries. whilst shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in iran, little is known about the genetic diversity of the local strains. therefore, th...
متن کاملDiversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated from Pediatric Patients
Multilocus variable number tandem repeat (VNTR) analysis (MLVA) is a new typing method with several advantages compared to other methods. Dissemination of Shigella is highly significant in developing countries. Whilst Shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in Iran, little is known about the genetic diversity of the local strains. Therefore, th...
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Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geogr...
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عنوان ژورنال:
gastroenterology and hepatology from bed to benchجلد ۸، شماره ۳، صفحات ۲۰۱۵-۸
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